American Samoa 2007). Central African Republic Belgium Bacillus spp. 5. Malta Nepal Plant Cell Physiol 53:497–507, Archana B, Saxena R (2012) Nematicidal effect of root extract of certain medicinal plants in control of J2 of Meloidogyne incognita in vitro and in vivo conditions. Mayotte Application of bio-arc (B. megaterium), nemastrol (a mixture of active ingredients), humisun (humic acid), dried sweet basil callus, and oxamyl singly or concomitantly in soil naturally infested with Meloidogyne spp. Saint Helena Morocco to assess the potential of bio-agents namely bio-arc (Bacillus megaterium), nemastrol (a mixture of active ingredients), humisun (humic acid), and dried sweet basil callus to suppress nematodes’ population and induce resistance in sugar beet. Bacillus spp. Guinea The inhibitory effect of Bacillus megaterium on aflatoxin and cyclopiazonic acid biosynthetic pathway gene expression in Aspergillus flavus. of species of the genus Bacillus, including B. licheniformis, B. subtilis, B. megaterium, and B. pumilus, were able to produce a lecithinase. Jamaica Nauru Czech Republic Trends Plant Sci 9:534–540, Radwan SM (1983) Effect of inoculation with phosphate dissolving bacteria on some nutrients uptake from newly cultivated soil, M.Sc Thesis. Burkina Faso Greece Italy Mauritania Article  Estonia It has the ability to kill the insects which are harmful for the crops, forests or agriculture. Japan's largest platform for academic e-journals: J-STAGE is a full text database for reviewed academic papers published by Japanese societies Thin-layer chromatography (TLC) and nuclear magnetic resonance (NMR) indicated the presence of high content of triterpenoides that belong to the three groups, i.e., lupane, ursane, and oleanane, in dried O. basilicum callus compared to native dried leaves powder. The authors declare that they have no competing interests. Iraq Vietnam Germany South Africa Single application of humisun (98%) and B. megaterium (58%) performed the best in ameliorating total plant fresh weight. Guadeloupe Meanwhile, O. basilicum callus showed more density content in rosmarinic acid than in dried leaves powder. Plant-growth-promoting rhizobacteria (PGPRs) offer an environment-friendly and efficient alternative to chemical pesticides and fertilizers. United States Minor Outlying Islands French Polynesia Privacy Cameroon Egypt: Faculty of Agriculture, Mansoura University Egypt; p 156, El-Nagdi WMA, Abd El Fattah AI (2011) Controlling root-knot nematode, Meloidogyne incognita infecting sugar beet using some plant residues, a biofertilizer, compost and biocides. The integration of B. megaterium with the nemastrol, humisun, dried sweet basil, and oxamyl induced systemic resistance towards the challenger Meloidogyne spp. Tunisia Other uses • a model organism for laboratory studies • a strain of B. subtilis formerly known as Bacillus natto is used in the commercial production of the Japanese food … Bermuda with strains of Bacillus megaterium and Bacillus muciaraglaginous together with AMF was associated with improved nutritional assimilation of plant total N, P, and K. Application of PGPR resulted in a significant increase in N, P Less branched roots were noticed with the introduction of sweet basil callus, sweet basil dried leaf powder, and oxamyl. Djibouti Therefore, nemastrol (chitinase12 × 105 IU) could be speculated to have a defense role during infection causing severe adverse effects on crucial biological processes of Meloidogyne spp. CAS  PubMed Google Scholar. Mexico East Timor Madagascar Cite this article. However, dried sweet basil callus showed better performance than did dried leaves in terms of female fecundity. Asian Pac J Trop Biomed 2(4):287–293. The data sets supporting the conclusions of this article are included within the article. Google Scholar, Duncan DB (1955) Multiple range and multiple, F-test. In agriculture, Bacillus megaterium can be used to produce phosphate-fixing and potassium-fixing fertilizers. Terms and Conditions, Macedonia infecting sugar beet. Agricultural Zoology Department, Faculty of Agriculture, Mansoura University, Mansoura, 11728, Egypt, Fatma A. M. Mostafa & Ahmed H. Nour El-Deen, Nematology Division, Plant Pathology Research Institute, ARC, Giza, Egypt, Present address: Biology Department, Faculty of Science, Taif University, Taif, Saudi Arabia, You can also search for this author in Israel i.e. However, sugar beet plants showed healthy storage root with NS + HS + BA + DSBC +O and NS + HS + BA as well. A field experiment, an area of 140 m2, with a randomized complete block design (RCBD) and replicated three times was practiced. It has a good effect of degrading organic phosphorus in soil. Niger (2016) reported that the Bacillus species, B. megaterium, B. cereus, and B. subtilis, produced cytokinins. San Marino Ecuador Bacilli are commonly used as plant growth-promoting agents but can be limited in effectiveness to certain crop and soil environments. Uzbekistan Amako A, Ghen GX, Asala K (1994) Separate assays specific for the ascorpate peroxides and guaiacol peroxidase and for the chloroplastic and cytosolic isozyme of ascorbate peroxidase in plants. Mostafa, F.A.M., Khalil, A.E., Nour El-Deen, A.H. et al. However, single application of dried sweet basil callus showed better performance than did the dried leaves in terms of female fecundity and total nematodes’ population. Therefore, attempts to increase active compounds in ornamental plants, i.e., sweet basil callus are needed for safe and effective nematodes’ management. CAS  Brunei in sugar beet. It can form spores. They can compound several kinds of vitamin group B like vitamin, niacin and etc., enhance the … https://doi.org/10.1186/s41938-018-0068-6, DOI: https://doi.org/10.1186/s41938-018-0068-6. French Guiana Oman Egypt: Faculty of Agriculture, Mansoura University; p 152, Nour El-Deen AH, Darwish HY (2011) Nematicidal activity of certain Egyptian weeds and bald cypress callus extracts against Meloidogyne incognita infecting eggplant under greenhouse conditions. Malawi One gram of dry weight of roots from each treatment was subjected to chemical analysis in order to evaluate total sugar solids (TSS), sucrose, and sugar purity. 2017). Ghana Eritrea Armenia Liberia Google Scholar, Coseteng MY, Lee CY (1987) Change in apple polyphenol oxidase and polyphenol concentrations in relation to degree of browning. Peru Google Scholar, Murashige T, Skoog F (1962) A revised medium for rapid growth and bio assays with tobacco tissue cultures. Egypt J Biol Pest Control 28, 66 (2018). A toolbox for recombinant protein production, secretion, and purification has been developed that is based on various vectors with different origins of replication, selection markers, promoters, affinity tag sequences, and sequences for a signal peptide. In vitro propagation was carried out in biotechnology laboratory at Nematological Research Unit (NERU), Agricultural Zoology Department, Faculty of Agriculture, Mansoura University, Egypt, to produce sterilized multiple shoots, the main source of callus (Murashige and Skoog 1962). It has a good effect of degrading organic phosphorus in soil. Taiwan Nematode population densities within 250 g soil and number of females (1 g /root) were significantly suppressed with single and concomitant applications by a reduction percentage in final nematode population ranged from 48.2 to 95.7% (Table 2). Iran Australia Egypt: Facuty of Agriculture, Mansoura University; p 157, Kesba HH, El-Beltagi HS (2012) Biochemical changes in grape rootstocks resulted from humic acid treatments in relation to nematode infection. Inorganic fertilizers, i.e., super phosphate15% (10 kg), was broadcasted, incorporated into the top of soil, and irrigated before planting. Papua New Guinea Kyrgyzstan It is used in the fight against many agricultural crop pests — mostly lepidopteran and coleopteran larvae — notably in the creation of new plant varieties expressing Bt cry genes. Saint Barthelemy Lecithinase is an enzyme that can disrupt the … J Plant Pathol Microbiol 5:236. https://doi.org/10.4172/2157-7471.1000236, CAS  on improving soil conditions, controlling plant diseases & increasing plant yields and quality. Romania Saint Lucia Falkland Islands D.J.W. By converting ineffective phosphorus into effective phosphorus, the absorption rate of phosphorus in the soil is effectively improved. Only logged in customers who have purchased this product may leave a review. Root galling (83.0%) and egg masses number (100.0%) were significantly suppressed for such treatment. Denmark Saudi Arabia Dominica Lithuania Springer Nature. Latvia Promote the growth of crops and prevent diseases. Christmas Island Dominican Republic Albania Antigua and Barbuda Costa Rica Saint Vincent and the Grenadines Switzerland B. cereus, B. megaterium, B. subtilis and B. stearothermophilus are available commercially ( Chakraborty et al., 2000 ; Das et al., 2004 ; Schallmey et al., 2004 ). Bacillus megaterium malachite-green staining Bacillus megaterium. Ireland Colombia Many researchers have investigated the antifungal and antimicrobial activities of certain plant callus extracts (Shariff et al. India Google Scholar, Rocha FS, Campos VP, Silva RC (2004) Culture of cells extract of several plants on second stage juveniles of Meloidogyne incognita. Lebanon Biological control and other eco-friendly disease control measures have gained increasing interest among researchers after the environmental restrictions on nematicidal use for controlling plant parasitic nematodes. This species is good cloning host because it is able to house numerous plasmid vectors while remaining stable due to its unique external proteases. Google Scholar, Gomez KA, Gomez AA (1984) Statistical procedures for agriculture research, 2nd edn. 2006), but little attention has been given to their use in nematode management (Rocha et al. Technological characters in terms of sucrose (17.1%), TSS (21.8%), and purity (79.0%) were significantly increased by introduction of the four bio-agents in concomitant with oxamyl (Table 3). Aspergillus flavus is one of the major moulds that colonize peanut in the field and during storage. Yemen Sao Tome and Principe Cyprus Afr J Biotechnol 6(11):1306–1316, CAS  are worldwide plant pathogens playing a detectable role in limiting the productivity of economic agriculture crops in temperate regions. Kong Q(1), Chi C, Yu J, … Previous studies reported that application of bio-arc + nemastrol under greenhouse conditions increased the activities of related enzymes, i.e., PO and PPO, in roots of sugar beet infected with M. incognita, as they reached their peaks at day 9 from nematode inoculation (Ibrahim 2013; Mostafa et al. Uganda Urea was reapplied after 2 weeks. The authors would like to thank to Dr. Hadeer Y. Darwish, Plant Res. 2007). Single application, spray application and flush application. The role of Bacillus megaterium and other bio-agents in controlling root-knot nematodes infecting sugar beet under field conditions. As fertilizer additive, use 1-2kg per ton fertilizer; 2. Mauritius by themselves, and play a role with digestive enzymes inside animals in the digestive tract. revealed that integrated of two or more components gave better results in sugar beet growth parameters than did single ones (Table 1). At each treatment, root hairs (1 g) were stained in 0.01 acid fuchsine lactic acid (Bybd et al. 2014). Utilization of biofertilizers based on the B. megaterium did not increase the number of Bacillus megaterium in the soil, but they were conducive to the stabilization of this strain in the soil environment that is crucial since any pressure affected on the homeostasis of soil environment has an adverse effect [ 50 ]. Chad Azerbaijan The presence of rosmarinic acid and terpenoids in methanolic extract of dried callus and powdered leaves of sweet basil was evaluated, using thin-layer chromatography (TLC) and nuclear magnetic resonance (NMR) (Kintzios et al. Nematol Bras 28(2):191–198, Salim HA, Salman SI, Majeed II, Hussein HH (2016) Evaluation of some plant extracts for their nematicidal properties against root-knot nematode, Meloidogyne sp. Gambia Tuvalu Norway The dietary supplementation of Bacillus spp. Tajikistan It can thicken the cell walls, fibrosis, and lignification of the relevant tissues of the plant and form a horny double silicon layer outside the epidermal layer to form a barrier to prevent the invasion of pathogens. About Press Copyright Contact us Creators Advertise Developers Terms Privacy Policy & Safety How YouTube works Test new features Japan Qatar Google Scholar, El-Deriny MM (2016) Integrated control of certain plant parasitic nematodes infecting cucurbitaceae plants, PhD Thesis. Manage cookies/Do not sell my data we use in the preference centre. Additionally, total plant fresh weight as well as shoot dry weight were obviously ameliorated by the application of NS + HS + BA + DSBC + O followed by NS + HS in concomitant with BA. Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations. Martinique Anguilla Herein, sweet basil callus (53.5%) and dried leaf powder (48.2%) exhibited the least reduction in nematodes’ population. Moriarty The University of Queensland, Australia INVE Aquaculture CAS  The use of screened organic materials, i.e., nemastrol, humisun, and sweet basil callus with the phosphorhizobacterium, B. megaterium, represents a promising new approach for the control of root-knot nematode, M. incognita, infecting sugar beet and enhances the resistance of plant to nematodes’ infection. https://doi.org/10.1016/S2221-1691(12)60024-0, Article  Philippines North Korea South Sudan Biota Max is remarkably versatile. Kiribati Tech Bull No.2 Min Agric. Belarus Greenland Pharmazie 60(1):62–65, PubMed  PubMed  Dora Agri is the leading supplier of Bacillus megaterium, it can be used to produce phosphate-fixing and potassium-fixing fertilizers. Results indicated that integration of two or more components of such bio-agents gave better results in sugar beet growth parameters than did single ones. Part of Pitcairn Iceland PubMed Central  Russia Results of this study support the findings of Mostafa et al. However, both enzymes showed less activities in the treatment of NS + HS + BA + DSBC +O than in the untreated plants. Sincere thanks are also given to Dr. Fatma Abdel Rahman, Faculty of Pharmacy, Mansoura University, Egypt, for the evaluation of rosmarinic acids and terpenoids in dried leaf callus of sweet basil using the thin-layer chromatography (TLC) and nuclear magnetic resonance (NMR). Cook Islands Uruguay Bouvet Island Bacillus spp. Guam Improve and extend fertilizer efficiency and reduce the use of chemical fertilizers. Montserrat Niue Arch Phytopathol Plant Protect 43:26–40, Ibrahim DS (2013) Induction of resistance to root-knot nematodes in sugar-beet plants, Ph D Thesis. B. megaterium plays an important role in dissolving the unavailable phosphorus compounds in soil rendering them available for growing crops (Radwan 1983). Rare to none: Bacillus megaterium is a rod-shaped, gram-positive, endospore forming, aerotolerant species of bacteria used as a soil inoculant in agriculture and ho ... Read More 2 doctors agree Argentina Plants were harvested 6 months after planting, and roots were washed free from adhering soil. Bahrain Zambia However, several plant species have been demonstrated to have nematicidal activity against plant parasitic nematodes (Salim et al. Seychelles Maldives Whilst growth parameters were significantly promoted (P ≤ 0.05) by the application of nemastrol and humisun in concomitant with bio-arc, sweet basil callus, and oxamyl (half recommended dose), such application induced significant (P ≤ 0.05) and maximum improvement in shoot (47.7%) and root (18.1%) lengths. infecting sugar beet under field conditions. Saint Pierre and Miquelon Bhutan Großkinsky et al. Antarctica volume 28, Article number: 66 (2018) After use, the dominant bacterial flora in the soil steadily maintains its dominant position. Next to B. subtilis, B. megaterium has attracted the most attention in recent years. Negma (three to four seeds/hill). Egypt It is supposed to act as a preformed constitutively accumulated defense compound (Gao et al. 2004). Cocos Islands Botswana Also humic acid treatments improved the yield of grape infected with M. incognita by increasing the activity of antioxidant enzymes (Kesba and El-Beltagi 2012). 2016; El-Deriny 2016; Khairy 2016). © 2020 BioMed Central Ltd unless otherwise stated. Callus tissues are among plant parts that have been evaluated for their nematicidal effects and introduced to pharmacological research tests (Rateb et al. Singapore Sint Maarten The activities of both peroxidase (PO) and polyphenol oxidase (PPO) showed detectable fluctuations at the end of the experiment compared to untreated plants. Rate of 0.3 g/plant in a single application of humisun ( 98 % ) potassium... Singly or concomitantly on Meloidogyne spp and egg masses under a stereomicroscope to the genus Bacillus this product leave! Rocha et al little attention has been given to their use in nematode management ( Rocha al! Leaves in terms of female fecundity springer Nature remains neutral with regard to claims. 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