Microscopic observations like shape and grams nature revealed the availability of similar morphological characters among isolates. Improvement in lentil breeding material, The Colorado Agricultural Experiment Station released 'Shiny Black Pearl' a black bean cultivar with shiny seed coat luster. Bean production constraints, bean seed quality and effect of intercropping on floury leaf spot disease and yields in Taita Taveta district, Kenya. Kalu District Office of Agriculture (KDOA) (2010). Pathogenicity testing of the obtained isolates were checked by artificial inoculation of leaves of mung bean using the methods described by Lelliott and Stead (1987) and Klement (1990). Among these pulses, chickpea is the major winter food legume and mung is the major summer legume. Sands DC (1999). Districts were selected purposively based on intensity of bean production and disease problems, while fields were randomly selected at intervals of about 5-10 km along the main and accessible road sides. At stage of vegetative mean incidence of the disease was observed as 45, 39 and 35% at Kewot, Kalu and Tehuledere districts respectively (Figure 2). Among these bacterial brown spot caused by Pseudomonas syringae pv. Cancer and dieback disease. Robert (2009) also reported the same finding with this result. Add more water if necessary just to keep the beans covered. Punjab is the major mung bean growing province that alone accounted for 88% area and 85% of the total mung bean … Plant Disease 101:95-102. The disease was noticed both at vegetative and flowering stages. Thus, it is necessary to investigate the geographic distribution of foliar devastating bacterial and disease to know the intensity and characterize the causal agent of the disease. Join ResearchGate to find the people and research you need to help your work. Khan A, Malik, MA (2001). The   intensity  of  disease  observed  also varied between districts and altitudes. Scheuermann et al. In Ethiopia, mung bean is a recent introduction and is found in different parts of the country (Teame et al., 2017). CSA (Central Statistical Agency) (2017). The Face Shop Real Nature Face Mask Mung Bean 1 Pc ... Korean Beauty Products Sheet Mask, Lip Tint, Cosmetics Cosrx, Tony Moly, Nature Republic, Innisfree Pakistan. Nutrient agar (NA) and nutrient broth (NB) were used as plating media (Lelliott and Stead, 1987). Lambrides C, Godwin ID (2007). Each of the factors may have a minor role in affecting disease. The mean severities were 15, 12 and 10% from districts of Kewot, Kalu and Tehuledere respectively (Figure 2). Positive association between bacterial brown spot epidemics and growth stage has been observed at flowering stage (Table 4). The mean prevalence of bacterial brown spot was 100 % in all districts surveyed at flowering stage (Figure 3). From the region, North Shewa and South Wollo are known to produce this crop. Simmer for another 15 to 20 minutes or until the beans … UI 906 (PI 549091) is a black seeded cultivar released by the University of Idaho (Myers et al. Abstract | Inqalab Mung (DM-3) was developed through cross between VC1482 C (AVRDC line Vigna cross, bold seeded, small stature and susceptible to Mungbean Yellow Mosac Virus (MYMV) and NM-92 (a promising variety of Pakistan, bold seeded, high yielding and MYMV resistant) at Agricultural Research Institute, Dera Ismail Khan. Mohammad G (2013). The scaling tests indicated the presence of non- allelic interactions for pod protein and, Genetic diversity is essential for any breeding program. On the affected leaves old lesion center fall out leaving shot holes was observed. The survey was done at two growth stages (vegetative and flowering) of the crop. The mean disease incidence of bacterial brown spot on mung bean showed 80.6, 83.3 and 74% at Kewot, Kalu, and Tehuledere districts respectively at flowering stage (Figure 2). Media and methods. According to this author, farmers in Ethiopia used mung bean as food and fodder (36.8%), income generation, food and fodder (32%), food, fodder and improve soil fertility (22.4%), food, fodder, improve soil fertility and income generation (8.8%). Aesculine medium containing plates was streaked by the test isolates and incubated at 20°C. Also, it is effectively cooked and does not cause flatulence (Pursglove, 2003). One hundred micro-liters of a 24 h. old bacterial suspension in nutrient broth was pipetted into a 3 mm diameter well on the center of each slice. Mwangombe A, Wagara I, Kimenju J, Buruchara R (2007). In this study, the genetic diversity of 21 common bean elite lines from the Bean Regional Trials. Based on genetic dissimilarity, three groups were defined: group I - lines 1, 9 and 10, with low genetic distances among them (0.00 to 0.06), originated from 11 Mesoamerican parents; group II - 17 lines with genetic distances ranging from 0.03 to 0.33, originated from 50 parents (mostly Mesoamerican); and group III - line 21 (PR 93201472), which parents are the Andean cultivar 'Pompadour' and the cultivar 'Irai' (unknown origin). Mung bean Mungbean is the most important kharif pulse of Pakistan and It is also grown during spring season mainly in southern Punjab and Sindh province. Asian Journal Plant Science 8:245-249. Methods of surveying the incidence and severity of cassava mosaic disease and whitefly vector populations on cassava in Africa: A review. This is the first report on the occurrence of such disease in Ethiopia. Ashrar M, Siririnives P, Sadiq MS, Saleem M (2001). © 2008-2020 ResearchGate GmbH. In: Laboratory guide for identification of plant pathogenic bacteria 2nd ed. Akademia Kiado, Budapest, Hungary, pp. Therefore, Shiny Black Pearl will require longer soak time to hydrate the seed prior to cooking than traditional opaque black bean cultivars. Vascular wilt of basin in Australia. The most serious fungal diseases which infect the mung bean are root rot (Macrophomina phaseolina), web blight, Rhizoctonia solani (Thanatephorus cucumeris), powdery mildew (Erysiphe polygoni), cercospora leaf spot (Cercospora canescens), anthracnose (Colletotrichum lindemuthianum) (Grewal, 1987). Identification of Bacterial Agent of the Bark Canker of Peach in the Province of Ilam. Soil condition relation with disease severity directed negative connection at flowering stage and vegetative stage (Table 4 and 5) that might be due the presence of dry soil condition during the assessment time for the spread of bacterial brown spot of mung bean. 4-6. Disease severity rating was evaluated on each plant using a modified 1 to 9 International Center for Tropical Agriculture scale, where 1 = 0% foliage affected, 3=2% foliage affected, 5 = 5% foliage affected, 7 = 10% foliage affected and 9 = 25% foliage affected (Van and Pastor-Corrales, 1987). (iv) It would be more interesting to study phenotypic characteristics of a large population of newly emerged Pss from various host plants in different regions of Ethiopia. © 2017 Pak. Offering a gamut of different flavours and textures, it will also fill you up thanks to mung beans. The amount of inoculum present in each farm, level of field sanitation and type of cropping system employed might also have influence on the overall disease distribution and epidemics (Stenglein et al., 2003; Mwangombe et al., 2007). Considering the proliferation of journals publishing food science and human nutrition related work, especially in the Third World countries, the actual number probably would be much higher. ECXA (2014). In: Virus and virus luke disease of major crops in developing countries, (Eds: Leobension D. and Thollapilly G.), Klieur Acadmaic Publisher, pp. Tween 80 was autoclaved separately and added with 10 ml/L and mixed before plating. RECRES 81A is agronomically and phenotypically similar to 81A, produces equally high yielding hybrids, and is being used to produce commercial hybrids. The plant selection that led to the development of Shiny Black Pearl was planted to a progeny row at Fort Collins CO, in 2002. The mung bean is native to Southwest Asia, where it was first cultivated 5,000-6,000 years ago. There are also several studies with regard to the inhibition potential of intercrops against different pathogens in various pathosystems (Chemeda, 2003; Altieri et al., 2005; Habtamu et al., 2015). (2004), by walking along in two diagonals (X-Fashion) of mung bean fields at spots of 0.5 m × 0.5 m (0.25 m2) quadrat. Itefa D (2016). phaseolicola) (Jitendra and Anila, 2018). Archives of Agronomy and Soil Science: Vol. The same result has been reported by Muedi et al. About (64%) of the adjacent crops observed in accordance with mung bean fields were cereals (Table 1). 74- 275. Hereditas 134:211-217. Each isolate were inoculated by stabbing and sealing with 3 ml sterilized molten agar to avoid false positives and were incubated at 28°C. Bacterial Diseases of Beans. Even more alarming are the trends of continued research on such aspects as the deficiency of sulfur-amino acids (both by amino acid analyses as well as rat feeding studies), and the heat lability/stability of proteinase inhibitors and phytohemagglutinins in various legume species. The production of typical symptoms for bacterial brown spot was observed under greenhouse condition of controlled environment. The typical leaf spot symptoms by all isolates were observed on leaves after eight days of inoculation. The reduction in photosynthetic activity and physiological changes are considerable, which leads to potential reduction in yield depending on the stage and time at which the diseases appears. However, not much variation was observed in the content of tannins and saponins. The biochemical tests positive reaction were to indicate the fluorescent Pseudomonas isolates. AVRDC Germplasm: Its utilization and development of improved mungbean.Pakistan Journal of Botany, p. 33. From mung-bean munching to being the Saudi Arabia of wind power generation, here's a summary of the UK PM's climate speech. Crossref. P ossible ways to increase area under Mung bean are inter-cropping with sugarcane crop, and sowing after harvesting of what. Although altitude showed negative relationship with intensities of disease at both growth stages, it was not significant (Tables 4 and 5). Avoid insect-damaged, broken, shriveled, discolored beans as they sound inferior quality. To test for the levan production of the isolates, a nutrient agar plates containing 5% sucrose were streaked by the test isolates and incubated for 3-5 days at 30°C. The planted pots were placed in the greenhouse with a temperature of 23 to 30°C. New Zealand Journal of Agricultural Research 23:267-272. Journal of Agricultural Science Technology 14:389-398. Schwartz et al. KOH test confirmed that all the isolates showed positive reaction and were categorized as gram negative rod shaped phytopathogenic bacteria. (2001) considered lower yield potential of mung bean is due to susceptibility to insect pests, diseases, undetermined excessive vegetative growth and small seed size. These two zones produce an average of 26277.54 tonnes annually. Madison, WI: University of Wisconsin. Pectate and pectin gel for differentiation of Pseudomonas sp. If environmental   conditions   are  known  to  favor  disease development, there might be high disease incidence and severity even in high altitude areas ranging 1625-2000 (Muedi et al., 2015). indexmarketsresearch December 14, 2020. The distribution of this destructive disease in all surveyed areas might be either due to the environmental conditions which promote developments of the disease and/or due to presence of diversified causative pathogen across different mung bean growing areas. When it result lower yields and poor grain qaulity. Locally mung bean is called as green gram. In  the  present  work,  we  have  performed  field  survey, observation  and  laboratory  diagnosis  to  investigate  the  status, isolate  and  identify  the  causal  pathogen. For H2S production surveyed at flowering stage ( Table 4 ) because of shading by the associate crop, is... Of Kalu varies from dry sub-humid to semi-arid ( on nutrient agar ( )... Other countries annual production like India 20.7 million tonnes and others ( FAO, 2017.. Anrs ) Bureau of Agriculture, Cali Colombia lesions may coalesce, out..., pp at pulses Research Institute, Ayub Agriculture Resea rice-wheat cropping system disease nurseries!, North Shewa and hence its consumption is not wide-spread like the pulses! To disease incidence and severity were recorded and used for identification of plant genetic diversity essential! 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